1-Hydroxybenzotriazole → tert-butyl 4-(7-(2-fluoro-4-(2-(4-fluorophenyl)-3-oxo-2,3-dihydropyridazine-4-carboxamido)phenoxy)thieno[3,2-b]pyridin-2-yl)-5,6-dihydropyridine-1(2H)-carboxylate

Participants

Reaction Steps

A round-bottomed flask was charged with tert-butyl 4-(7-(4-amino-2-fluorophenoxy)thieno[3,2-b]pyridin-2-yl)-5,6-dihydropyridine-1(2H)-carboxylate (30.0 mg, 0.068 mmol), 2-(4-fluorophenyl)-3-oxo-2,3-dihydropyridazine-4-carboxylic acid (Example 104, Step C, 31.8 mg, 0.136 mmol), N1-((ethylimino)methylene)-N3,N3-dimethylpropane-1,3-diamine hydrochloride (39.1 mg, 0.204 mmol), 1H-benzo[d][1,2,3]triazol-1-ol (27.5 mg, 0.204 mmol), N-ethyl-N-isopropylpropan-2-amine (0.061 ml, 0.340 mmol) and DMF (5 mL). The reaction mixture was stirred at room temperature for 48 hours. Then the reaction was partitioned between EtOAc (50 mL) and H2O (50 mL). The phases were separated and the aqueous phase was re-extracted with EtOAc (3×100 mL). The combined organic layers were dried (Na2SO4), filtered and concentrated to yield a crude product. The crude product was purified by silica gel chromatography (DCM/7 M NH3 in MeOH from 100/1 to 10/1, v/v) to afford product (27.3 mg, 61.1%). LRMS (APCI pos) m/e 658 (M+1). 1H NMR (400 MHz, CDCl3) δ 11.82 (s, 1H), 8.42 (m, 2H), 8.24 (d, 1H), 7.98 (dd, 1H), 7.21-7.66 (m, 7H), 6.58 (s, 1H), 6.30 (m, 1H), 4.18 (m, 2H), 3.68 (m, 2H), 2.62 (m, 2H), 1.52 (s, 9H).

Provenance & Evidence