Sodium Chloride → Citrulline

Participants

Reaction Steps

IC50 values were determined by pre-incubating various amounts of the potential inhibitor in Reaction Buffer (10 mM CaCl2, 2 mM DTT, 50 mM NaCl, 100 mM Tris HCl pH 7.6) and 0.2 μM PAD4 at 37° C. After 15 minutes, Benzoyl Arginine Ethyl Ester (BAEE; 1 mM final; 0.74×Km) was added and the reaction was allowed to proceed for 15 minutes (60 μL total volume). Reactions were quenched by flash freezing in liquid N2. The amount of Cit produced was then quantified according to methods known in the art. IC50 values were determined by fitting the data to equation 1, FracionalActivity=1/(1+[I]/IC50)   (1) using GraFit version 5.0.11. Inhibition Studies.

Provenance & Evidence