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HRID1676880

反应详情

EQUATION

反应方程式

HRID 1676880 的结构方程式

REACTANTS

反应物

4
结构：CCOC(=O)[C@H](CCCN=C(N)N)NC(=O)c1ccccc1
HCID123880

Ethyl N-benzoyl-L-arginine

C15H22N4O3

结构：CN(C)c1ccc(O)c2c1C[C@H]1C[C@H]3[C@H](N(C)C)C(O)=C(C(N)=O)C(=O)[C@@]3(O)C(O)=C1C2=O
HCID178482881

未命名化合物

结构：CN(C)[C@@H]1C(O)=C(C(N)=O)C(=O)[C@@]2(O)C(O)=C3C(=O)c4c(O)ccc(Cl)c4[C@@](C)(O)[C@H]3C[C@@H]12
HCID178484163

未命名化合物

结构：CN[C@@H]1[C@H](O[C@H]2[C@H](O[C@H]3[C@H](O)[C@@H](O)[C@H](NC(=N)N)[C@@H](O)[C@@H]3NC(=N)N)O[C@@H](C)[C@]2(O)C=O)O[C@@H](CO)[C@H](O)[C@H]1O
HCID178484872

未命名化合物

PRODUCTS

生成物

1
结构：NC(=O)NCCC[C@H](N)C(=O)O
HCID9750

Citrulline

C6H13N3O3

PROCEDURE

实验过程

Inhibition constants were determined for minocycline, chlortetracycline, and streptomycin using standard kinetic analyses. Briefly, the compounds were incubated in Reaction Buffer containing various concentrations of BAEE (0-20 mM) for 10 minutes at 37° C. PAD4 (0.2 μM) was then added to initiate the reaction and allowed to proceed for 6 additional minutes. Reactions were then quenched by flash freezing in liquid N2 and the amount of Cit produced was quantified. The data obtained for streptomycin, minocycline and chlortetracycline were fit to equations 2 and 3,

WORKUP

后处理

  1. additionPAD4 (0.2 μM) was then added
  2. customthe reaction
  3. customReactions were then quenched by flash