Hydrochloric Acid → HCID 59023721, compound

Participants

Reaction Steps

A solution of {[5-(2-amino-pyrimidin-4-ylamino)-1H-indazole-3-carbonyl]-amino}acetic acid methyl ester (was prepared from Example N.2.8, B, 0.014 g, 0.043 mmol) and 2M aqueous NaOH solution (1.0 mL, 2.0 mmol) in 1,4-dioxane (2 mL) was stirred at ambient temperature for about 30 minutes. The reaction mixture was acidified with 1M aqueous HCl solution until pH 3. The white precipitation was filtered off, washed with water (3 mL) and dried under reduced pressure to give {[5-(2-amino-pyrimidin-4-ylamino)-1H-indazole-3-carbonyl]-amino}-acetic acid (0.001 g, 0.003 mmol); RP HPLC (Table 1, Method a) Rt=0.42 min; m/z: (M+H)+ 328.2.

Provenance & Evidence