Dichloromethane → HCID 59023793, compound

Temperature

90 °C

[1,1′-Bis(diphenylphosphino)-ferrocene)dichloropalladium(II) 1:1 complex with dichloromethane (408 mg, 0.50 mmol) was added to a mixture of methyl 5-bromo-1-(4-methoxy-benzyl)-1H-indazole-7-carboxylate ester and methyl 5-bromo-2-(4-methoxy-benzyl)-2H-indazole-7-carboxylate (Preparations #11 and 12, 1:1, 3.99 g, 9.45 mmol), cesium carbonate (9.75 g, 30.0 mmol) and N′-(1-benzenesulfonyl-3-bromo-1H-pyrrolo[2,3-c]pyridin-5-yl)-N,N-dimethyl-formamidine (Preparation #10, 3.85 g, 9.45 mmol) in 1,2-dimethoxyethane (100 mL) and water (25 mL) under an atmosphere of N2. The reaction mixture was heated at about 90° C. for about 4 hours. The reaction was cooled to ambient temperature and the layers separated. The organic layer was filtered through a silica gel pad, eluting with 5% MeOH/EtOAc and the filtrate was concentrated under reduced pressure. The residue was dissolved in trifluoroacetic acid (50 mL) containing triisopropylsilane (2.05 ml, 10.0 mmol) and the mixture was heated in a sealed tube at about 100° C. for about 30 minutes then cooled to ambient temperature and concentrate under reduced pressure. The residue was triturated with ether (100 mL) and the resulting solid was collected by filtration. The solid was then dissolved in methanol (75 mL), treated with aqueous sodium hydroxide (2N, 50 ml, 100 mmol) and heated at reflux for about 6 hours. The reaction was filtered hot, then cooled and acidified with acetic acid to about pH 5.5. The product was filtered off, washed with water (2×10 mL) and dried to yield 5-(5-amino-1H-pyrrolo[2,3-c]pyridin-3-yl)-1H-indazole-7-carboxylic acid (2.60 g, 78%) as the acetate salt. The acetate salt was dissolved in methanol (300 mL) and aqueous HCl (2N, 25 mL) was added. The mixture was concentrated and dried to yield 5-(5-amino-1H-pyrrolo[2,3-c]pyridin-3-yl)-1H-indazole-7-carboxylic acid monohydrochloride (2.35 g, 75%) as a pale yellow solid. RP HPLC (Table 1, Method e) Rt=1.31, m/z: (MH)+ 294.

1. temperatureThe reaction was cooled to ambient temperature
2. customthe layers separated
3. filtrationThe organic layer was filtered through a silica gel pad
4. washeluting with 5% MeOH/EtOAc
5. concentrationthe filtrate was concentrated under reduced pressure
6. dissolutionThe residue was dissolved in trifluoroacetic acid (50 mL)
7. temperaturethe mixture was heated in a sealed tube at about 100° C. for about 30 minutes
8. temperaturethen cooled to ambient temperature
9. concentrationconcentrate under reduced pressure
10. customThe residue was triturated with ether (100 mL)
11. filtrationthe resulting solid was collected by filtration
12. dissolutionThe solid was then dissolved in methanol (75 mL)
13. temperatureheated
14. temperatureat reflux for about 6 hours
15. filtrationThe reaction was filtered hot
16. temperaturecooled
17. filtrationThe product was filtered off
18. washwashed with water (2×10 mL)
19. customdried

ORD record

ord-caae72217a8040ecba3cdf051ea38ca4

Source dataset

uspto-grants-2011_08

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US08008481B2