Phenol → HCID 22138231, compound

Participants

Reaction Steps

1.0 ml of a solution to be tested was charged in a test tube, and 1.0 ml of a 5% aqueous solution of phenol was added and the mixture was agitated so as to become homogeneous. Then, 0.5 ml of concentrated sulfuric acid was added to the mixture and reaction was conducted under agitation. The reaction mixture was allowed to stand still for 10 minutes and then was cooled with water. The absorbance at 489 mμ was measured, and a determination was conducted based on a calibration curve obtained by treating an aqueous solution of a known amount of dextran in the same manner as described above.

Provenance & Evidence