Water → N-(tert-Butoxycarbonyl)-L-tyrosyl-D-methionylglycine

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Reaction Steps

6.02 Grams of methyl Boc-L-tyrosyl-D-methionyl-glycinate is dissolved in 30 ml of methyl alcohol and water is added until the solution becomes cloudy. A 4 M solution of potassium hydroxide is added to maintain the pH at 12-13. When the pH is stable at 13 for 30 minutes, the methanol is removed under reduced pressure. The aqueous solution is filtered and adjusted to pH 3 with concentrated hydrochloric acid. The product crystallizes and is removed by filtration and washed with water to give Boc-L-tyrosyl-D-methionyl-glycine which is represented by the following formula

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