反应详情

(2S,4R)-4-(2-Chloro-benzenesulfonyl)-1-[5-methyl-2-(4-trifluoromethyl-phenyl)-2H-pyrazol-3-yl]-pyrrolidine-2-carboxylic acid methyl ester (27 mg, 51 umol) was dissolved in THF (0.2 ml). A solution of lithium hydroxide monohydrate (3 mg, 72 umol) in water (0.2 ml) was added and the mixture was stirred at ambient temperature for 14 h. Ice water/0.1 N aqueous HCl solution 1/1 and iPrOAc were added and the layers were separated. The aqueous layer was extracted one more time with iPrOAc, the combined organic layers were washed with ice water/brine 1/1 and dried over Na2SO4. The solvent was removed under reduced pressure to give the title compound compound (26 mg, 51 umol; quant.) as a yellow oil which was used in the next step without further purification. MS (ESI): m/z=514.3 [M+H]+.

1. customthe layers were separated
2. extractionThe aqueous layer was extracted one more time with iPrOAc
3. washthe combined organic layers were washed with ice water/brine 1/1
4. dry with materialdried over Na2SO4
5. customThe solvent was removed under reduced pressure