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HRID1703536

反应详情

EQUATION

反应方程式

HRID 1703536 的结构方程式

REACTANTS

反应物

6
结构：[Cl-].[Na+]
HCID5234

Sodium chloride

ClNa

结构：O=C([O-])CC(O)(CC(=O)[O-])C(=O)[O-].[Na+].[Na+].[Na+]
HCID6224

Trisodium citrate

C6H5Na3O7

结构：O=P([O-])([O-])[O-].[K+].[K+].[K+]
HCID62657

Tripotassium phosphate

K3O4P

结构：Nc1ccn([C@H]2C[C@H](O)[C@@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O2)c(=O)n1
HCID65091

2′-Deoxycytidine 5′-(tetrahydrogen triphosphate)

C9H16N3O13P3

结构：[Cl-].[Cl-].[Mg+2]
HCID5360315

Magnesium Chloride

Cl2Mg

结构：O=c1nc[nH]c2c1ncn2[C@H]1C[C@H](O)[C@@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O1
HCID178518211

未命名化合物

PRODUCTS

生成物

1
结构：Nc1ccn([C@H]2C[C@H](O)[C@@H](COP(=O)(O)O)O2)c(=O)n1
HCID13945

2′-Deoxycytidine-5′-monophosphate

C9H14N3O7P

CONDITIONS

反应条件

温度
-20 °C

PROCEDURE

实验过程

A reaction mixture for the synthesis of poly (dI:dC) contains 3.5 μ moles each of dITP and deoxycytidine-5'-triphosphate (dCTP), 600 μ moles of potassium phosphate buffer (pH 7.4) and 30 μ moles of magnesium chloride, and 40-80 units of DNA (deoxynucleic acid) polymerase in a total volume of 10 ml. After a lag period of 2-4 hours, polymerization is complete in 6-8 hours. The course of the reaction is followed by optical density measurements at intervals. When hypochromicity at 260 mμ is at a maximum, the reaction is stopped by adjusting the mixture to 0.2M sodium chloride and 0.1M sodium citrate. Dialysis is extensively carried out against the same buffer to remove all low molecular weight material. The product is stored frozen at -20° C. until used for interferon induction.

WORKUP

后处理

  1. customA reaction mixture for the synthesis of poly (dI:dC)
  2. customAfter a lag period of 2-4 hours, polymerization
  3. customDialysis
  4. customto remove all low molecular weight material