HCID 131711777, compound → HCID 131711776, compound

Participants

Reaction Steps

In 3 ml of 5% thioglycolic acid-50% aqueous acetic acid was dissolved 30 mg of H-Arg-Arg-Ala-Gln-Asp-Phe-Val-Gln-Trp-Leu-Met(O)-Asn-Thr-OH and reduction was carried out at 45° C. for 24 hours. Then, the reaction mixture was run onto a column (2.4×122 cm) of Sephadex G-25 and elution was carried out with 30% aqueous acetic acid. The fractions from 180 ml to 250 ml were pooled and lyophilized. Yield 29 mg; [α]D21 -41.9° (c=0.4%, 0.1 N-HCl); Rf3 0.55(Avicel®); amino acid analysis: Trp 0.69(1); Arg 2.25(2); Asp 2.13(2); Thr 0.97(1); Glu 2.16(2); Ala 1.00(1); Val 1.01(1); Met 0.97(1); Leu 1.01(1); Phe 1.05(1); (peptide content 78%)

Provenance & Evidence