HCID 131711780, compound → Glucagon (19-29)

Participants

Reaction Steps

In 3.0 ml of 5% thioglycolic acid-50% aqueous acetic acid was dissolved 30 mg of H-Ala-Gln-Asp-Phe-Val-Gln-Trp-Leu-Met(O)-Asn-Thr-OH and reduction was carried out at 45° C. for 24 hours. The reaction mixture was run onto a column (3.4×57 cm) of Sephadex LH-20 and elution was carried out with 50% acetic acid. The fractions from 235 ml to 280 ml were pooled and lyophilized. Yield 23 mg; Rf3 0.72(Avicel®); amino acid analysis; Trp (0.76(1); Asp 2.15(2); Thr 0.93(1); Glu 2.15(2); Ala 0.97(1); Val 1.00(1); Met 0.92(1); Leu 1.02 (1); Phe 1.02(1) (peptide content 82%)

Provenance & Evidence