Sodium Chloride → Dopamine

Participants

Reaction Steps

0.5 mL aliquots of the membrane preparation were incubated with unlabeled drugs, and 0.15 nM [3H]spiperone in a final volume of 1 mL containing 50 mM Tris HCl, 120 mM NaCl and 1 mM MgCl2 (pH 7.7). Nonspecific binding was measured in the presence of 100 nM (+)-butaclamol After 15 min of incubation at 37° C., samples were filtered rapidly through Whatman GF/C glass filters under negative pressure, and washed three times with ice-cold binding buffer (5 mL). Data are shown in Table I (+++, Ki 1-30 nM; ++, Ki 31-100 nM; +, Ki 101-500 nM; -, Ki >500 nM).

Provenance & Evidence