Eugenol → Vanillin

Participants

Reaction Steps

On the basis of the substrates (a) Benzoe Siam Resin (b) isoeugenol and (c) eugenol, all dispersed in tap water as the reaction medium, the compound vanillin was produced. The substrates were taken up in flasks mounted on a shaking apparatus after which the enzyme lipoxidase (Sigma L8383) in a dose of 0.2% w/w based on the substrate was added. The temperature during the reaction was kept at 25° C. The starting mixture had a initial pH of 8. After an incubation time of 24 hours the reaction was stopped by inactivation of the enzyme (10 min. at 70° C.). The results of the experiments are shown in Table B below.

Provenance & Evidence