Tert-Butyl nitrite → HCID 11294280, compound

Participants

Reaction Conditions

Reaction Steps

To a solution of Example 20 (150 mg, 0.31 mmol) in tetrahydrofuran (5 ml) was added dropwise tert-butyl nitrite (111 μl, 0.93 mmol). The reaction mixture was then heated at 60° C. overnight. The reaction mixture was concentrated in vacuo and the residue was partitioned between ethyl acetate (20 ml) and water (20 ml). The organic layer was separated, washed with brine (20 ml), dried (Na2SO4) and concentrated in vacuo. The residue was purified by column chromatography (silica, 10 g) eluting with dichloromethane/ethyl acetate [9:1]. The appropriate fractions were combined and concentrated to give the crude product. The crude product was dissolved in a mixture of acetonitrile, dimethyl sulphoxide and water (4:5:1, 2 ml) and purified by automated preparative liquid chromatography (Gilson system, 150 mm×30 mm Phenomonex LUNA C18(2) 10 μm column) using an acetonitrile:water gradient [60:40 to 95:5]. The appropriate fractions were concentrated in vacuo to give the titled compound (80 mg).

Provenance & Evidence