Cholesterol → Cholesterol

Participants

Reaction Steps

The optimum pH for the production of cholestenone when epicholesterol is used as the substrate, is 8-12. The optimum pH when epicholesterol is produced from 5-cholesten-3-one, is 4-5. Determined at 37° C. using various pH buffer, 0.1M acetate/hydrochloride buffer (pH 2-4), 0.1M phosphate/citrate buffer (pH 4-7), 0.1M Tris-HCl buffer (pH 7-9), and 0.1M glycine/sodium hydroxide buffer (pH 9-12), each containing 1 mM dithiothreitol!.

Provenance & Evidence