Sodium Hydroxide → L-Tryptophan

HRID 1728911Notes

Reaction equation

HRID 1728911 reaction equation
Cl.[NH2:2][C@H:3]([C:14]([OH:16])=[O:15])[CH2:4][C:5]1[C:13]2[C:8](=[CH:9][CH:10]=[CH:11][CH:12]=2)[NH:7][CH:6]=1.[OH-].[Na+]>>[NH2:2][C@H:3]([C:14]([OH:16])=[O:15])[CH2:4][C:5]1[C:13]2[C:8](=[CH:9][CH:10]=[CH:11][CH:12]=2)[NH:7][CH:6]=1

Participants

3

Reactant 2

HCID 14798Sodium HydroxideHCID 134608Tryptophan--hydrogen chloride (1/1)2×

Product 1

HCID 6305L-Tryptophan

Procedure

An aqueous solution of 130 g of the L-tryptophan hydrochloride crystals at a concentration of 150 g/l and 27% aqueous sodium hydroxide solution were simultaneously fed into a crystallization tank at 30° C., while controlling the feeding rate of each solution so that the pH of the crystallization slurry was maintained at 6.5. After addition of the solution of L-tryptophan hydrochloride was completed, the crystallized L-tryptophan was isolated with a centrifuging machine. The crystals were then washed with water, and dried at 60° C. for 2 hours under reduced pressure to give 92 g of L-tryptophan crystals. The L-tryptophan crystals thus obtained had a purity of 96.4%, corresponding to a yield of 75% of L-tryptophan from that produced in the fermentation broth.

Workup

  1. customwere simultaneously fed into a crystallization tank at 30° C.
  2. temperaturewas maintained at 6.5

Sources & evidence

ORD record
ord-c4bc33fef738415d8d9ba98af8f2e883
Source dataset
uspto-grants-1994_07
Patent number
US05329014

Reaction notes

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