Hydrochloric Acid → HCID 131714969, compound

Participants

Reaction Steps

A solution of 7-[2-methoxyimino-2-{2-(2,2,2-trifluoroacetamido)thiazol-4-yl}acetamido]-3-vinylthiomethyl-3-cephem-4-carboxylic acid (syn isomer) (380 mg) and sodium acetate (937 mg) in water (8 ml) was stirred at ambient temperature overnight. The mixture was adjusted to pH 1.6 with 6N-hydrochloric acid. This solution was subjected to column chormatography on macroporous non-ionic adsorption resin "Diaion HP 20" (15 ml). After the column was washed with water, the elution was carried out with 25% aqueous isopropyl alcohol. The eluates containing the object compound were collected, evaporated to remove isopropyl alcohol under reduced pressure and lyophilized to give 7-[2-(2-aminothiazol-4-yl)-2-methoxyiminoacetamido]-3-vinylthiomethyl-3-cephem-4-carboxylic acid hydrochloride (syn isomer) (170 mg).

Provenance & Evidence