Phosphate → Penicillanic Acid

HRID 1732905Notes

Reaction equation

HRID 1732905 reaction equation
[CH3:1][C:2]1([CH3:14])[S:6][C@@H:5]2[C@H:7](N)[C:8](=[O:9])[N:4]2[C@H:3]1[C:11]([OH:13])=[O:12].P([O-])([O-])([O-])=O.N=C=N>O>[CH3:1][C:2]1([CH3:14])[S:6][C@@H:5]2[CH2:7][C:8](=[O:9])[N:4]2[C@H:3]1[C:11]([OH:13])=[O:12]

Participants

5

Reactant 3

HCID 1061PhosphateHCID 110826-Aminopenicillanic acid2×HCID 160435Carbodiimide

Product 1

HCID 6891Penicillanic Acid

Reagents, catalysts & solvents 1

HCID 962WaterSolvent

Conditions

Temperature
4 °C

Procedure

A conjugate of penicillanic acid and peroxidase was prepared as follows. An alkaline solution (pH=9.0) containing 28.1 mg 6 amino penicillanic acid is mixed with a phosphate buffered peroxidase solution containing 8.8 mg peroxidase and 8.2 mg carbodiimide in water. The reaction mixture is maintained at 4° C. for 24 hours while stirring. As a result, the peroxidase and 6 amino penicillanic acid became covalently linked. The conjugate solution is next dialyzed against 0.05 M phosphate buffered saline (PBS); penicillin activity disappears from the PBS after the first change of solution. After four solution changes, the penicillin-peroxidase conjugate is ready for use.

Workup

  1. additioncontaining 8.8 mg peroxidase
  2. customnext dialyzed against 0.05 M phosphate buffered saline (PBS)

Sources & evidence

ORD record
ord-1034b6c792894bdd9c3e08717bdb5198
Source dataset
uspto-grants-1980_12
Patent number
US04239852

Reaction notes

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