Ammonium → 2-Amino-5-[glycyl(methyl)amino]-3,4,6-trihydroxycyclohexyl 2,6-diamino-2,3,4,6,7-pentadeoxyheptopyranoside

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Reaction Conditions

Reaction Steps

Fortimicin A sulfate (1.48 g, 2.46 mmole) was dissolved in 160 ml of 48% hydriodic acid at room temperature. The solution was stirred and the temperature gradually raised to 62° C. Stirring was continued at 62° C. for 20 hours. The solution was diluted with 160 ml of water and passed through a 1.5 l. column of "Amberlite" lRC-50 in the ammonium form. The column was washed with water and eluted with an ammonium hydroxide gradient as described in Example 1 resulting in the recovery of 3-O-demethylfortimicin A in 25% yield.

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