反应详情

The procedure described in Comparative Example A was repeated using a 10 mL aqueous solution containing sodium L-lactate (0.500M), isobutyric acid (HPLC internal standard, 0.100M), soluble spinach glycolate oxidase (2.0 IU/mL), and soluble Aspergillus niger catalase (20,000 IU/mL) at pH 9.0 (adjusted with 50% NaOH), and the reaction run at 15° C; no buffer was added. After 7 hours, the HPLC yields of pyruvate and acetate were 91.6% and 0.6%, respectively, and 7.1% lactate remained. The remaining activities of glycolate oxidase and catalase were 21% and 100%, respectively, of their initial values.

1. customat 15° C
2. additionno buffer was added