Isobutyric Acid → Acetate

HRID 1735657Notes

Reaction equation

HRID 1735657 reaction equation
[C:1]([O-:6])(=[O:5])[C@H:2]([CH3:4])[OH:3].[Na+].[C:8]([OH:13])(=[O:12])[CH:9](C)C.C([O-])(=O)CO.[OH-].[Na+]>>[C:1]([O-:6])(=[O:5])[C:2]([CH3:4])=[O:3].[C:8]([O-:13])(=[O:12])[CH3:9]

Participants

6

Reactant 4

HCID 6590Isobutyric AcidHCID 14798Sodium HydroxideHCID 5460308HydroxyacetateHCID 23664767Sodium Lactate, L-

Product 2

HCID 175AcetateHCID 107735Pyruvate

Procedure

The procedure described in Comparative Example A was repeated using a 10 mL aqueous solution containing sodium L-lactate (0.500M), isobutyric acid (HPLC internal standard, 0.100M), soluble spinach glycolate oxidase (2.0 IU/mL), and soluble Aspergillus niger catalase (20,000 IU/mL) at pH 9.0 (adjusted with 50% NaOH), and the reaction run at 15° C; no buffer was added. After 7 hours, the HPLC yields of pyruvate and acetate were 91.6% and 0.6%, respectively, and 7.1% lactate remained. The remaining activities of glycolate oxidase and catalase were 21% and 100%, respectively, of their initial values.

Workup

  1. customat 15° C
  2. additionno buffer was added

Sources & evidence

ORD record
ord-eeb6c0867c4f450abee6a397761fef26
Source dataset
uspto-grants-1996_07
Patent number
US05538875

Reaction notes

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