Isobutyric Acid → Acetate

Participants

Reaction Steps

The procedure described in Comparative Example A was repeated using a 10 mL aqueous solution containing L-lactic acid (96% L-isomer, 4% D-isomer, 0.500M), KH2PO4 (0.50M), FMN (0.01 mM), isobutyric acid (HPLC internal standard, 0.100M), soluble spinach glycolate oxidase (2.0 IU/mL), and soluble Aspergillus niger catalase (14,000 IU/mL) at pH 8.3 (adjusted with 50% NaOH), and the reaction run at 5° C. After 18 hours, the HPLC yields of pyruvate and acetate were 90.5% and 4.2%, respectively, and 6.4% lactate remained. The remaining activities of glycolate oxidase and catalase were 57% and 100%, respectively, of their initial values.

Provenance & Evidence