Tris(hydroxymethyl)aminomethane → Acetate

Participants

Reaction Steps

The procedure described in Comparative Example A was repeated using a 10 mL aqueous solution containing L-lactic acid (96% L-isomer, 4% D-isomer, 0.750M total), FMN (0.01 mM), isobutyric acid (HPLC internal standard, 0.100M), Tris buffer (0.750M), soluble spinach glycolate oxidase (1.0 IU/mL), and soluble Aspergillus niger catalase (1,400 IU/mL) at pH 8.3 (adjusted with 50% NaOH) and the reaction run at 5° C. After 39 hours, the HPLC yields of pyruvate and acetate were 55.1% and 6.3%, respectively, and 2.7% lactate remained. The remaining activity of glycolate oxidase and catalase were 29% and 94%, respectively, of their initial values.

Provenance & Evidence