反应详情

The procedure described in Example 1 was repeated using 10 mL of an aqueous solution containing sodium L-lactate (0.500M) and isobutyric acid (HPLC internal standard, 0.100M) at pH 9.0 (adjusted with 50% NaOH), to which was added 0.75 g (wet weight) of Pichia pastoris transformant GS115-MSP 10 (6.52 IU/mL glycolate oxidase and 10,100 IU/mL catalase) which had been permeabilized by treatment with 0.1% benzalkonium chloride ("BARQUAT" OJ-50); no buffer was added. After 5 hours, the HPLC yields of pyruvate and acetate were 99.0% and 0.7%, respectively, and 0.4% lactate remained. The remaining permeabilized cell activity of glycolate oxidase and catalase were 119% and 113%, respectively, of their initial values.

1. additionno buffer was added