Isobutyric Acid → Acetate

HRID 1735664Notes

Reaction equation

HRID 1735664 reaction equation
[C:1]([O-:6])(=[O:5])[C@H:2]([CH3:4])[OH:3].[Na+].[C:8]([OH:13])(=[O:12])[CH:9](C)C.[OH-].[Na+].C([O-])(=O)CO.CCCCCCCCCCCCC[N+](CC1C=CC=CC=1)(C)C.[Cl-]>>[C:1]([O-:6])(=[O:5])[C:2]([CH3:4])=[O:3].[C:8]([O-:13])(=[O:12])[CH3:9]

Participants

7

Reactant 5

HCID 6590Isobutyric AcidHCID 14798Sodium HydroxideHCID 3014024Benzalkonium ChlorideHCID 5460308HydroxyacetateHCID 23664767Sodium Lactate, L-

Product 2

HCID 175AcetateHCID 107735Pyruvate

Procedure

The procedure described in Example 1 was repeated using 10 mL of an aqueous solution containing sodium L-lactate (0.500M) and isobutyric acid (HPLC internal standard, 0.100M) at pH 9.0 (adjusted with 50% NaOH), to which was added 0.75 g (wet weight) of Pichia pastoris transformant GS115-MSP 10 (6.52 IU/mL glycolate oxidase and 10,100 IU/mL catalase) which had been permeabilized by treatment with 0.1% benzalkonium chloride ("BARQUAT" OJ-50); no buffer was added. After 5 hours, the HPLC yields of pyruvate and acetate were 99.0% and 0.7%, respectively, and 0.4% lactate remained. The remaining permeabilized cell activity of glycolate oxidase and catalase were 119% and 113%, respectively, of their initial values.

Workup

  1. additionno buffer was added

Sources & evidence

ORD record
ord-9c8b6f3bcbb04ddebff395a410a28868
Source dataset
uspto-grants-1996_07
Patent number
US05538875

Reaction notes

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