Isobutyric Acid → Acetate

Participants

Reaction Steps

The procedure described in Example 1 was repeated using 10 mL of an aqueous solution containing sodium L-lactate (1.00M) and isobutyric acid (HPLC internal standard, 0.100M) at pH 9.0 (adjusted with 50% NaOH), to which was added 0.71 g (wet weight) of Pichia pastoris transformant GS115-MSP10 (4.59 IU/mL glycolate oxidase and 10,000 IU/mL catalase) which had been permeabilized by treatment with 0.1% benzalkonium chloride ("BARQUAT" OJ-50); no buffer was added. After 8 hours, the HPLC yields of pyruvate and acetate were 89.1% and 8.4%, respectively, and 1.3% lactate remained. The remaining permeabilized cell activity of glycolate oxidase and catalase were 124% and 145%, respectively, of their initial values.

Provenance & Evidence