Oxygen → Acetate

Participants

Reaction Steps

The procedure described in Example 7 was repeated using 10 mL of an aqueous solution containing sodium L-lactate (0.50M) and isobutyric acid (HPLC internal standard, 0.100M) at pH 9.0 (adjusted with 50% NaOH), to which was added 1.04 g (wet weight) of Hansenula polymorpha transformant GO1 (6.47 IU/mL glycolate oxidase and 5,000 IU/mL catalase) which had been permeabilized by treatment with 0.1% benzalkonium chloride ("BARQUAT" OJ-50); no buffer was added. The reaction temperature was 15° C. and the oxygen pressure was 70 psig. After 2 hours, the HPLC yields of pyruvate and acetate were 97.0% and 2.5%, respectively, and 0.4% lactate remained. The remaining permeabilized cell activity of glycolate oxidase and catalase were 99% and 155%, respectively, of their initial values.

Provenance & Evidence