Oxygen → Acetate

Participants

Reaction Steps

The procedure described in Example 11 was repeated using 100 mL of an aqueous solution containing sodium L-lactate (0.713M) at pH 7.5 (adjusted with 50% NaOH), to which was added 5.00 g (wet weight) of Pichia pastoris transformant GS115-MSP10 (3.27 IU/mL glycolate oxidase and 10,200 IU/mL catalase) which had been permeabilized by treatment with 0.1% benzalkonium chloride ("BARQUAT" MB-50); no buffer was added. The reaction temperature was 5° C. and the oxygen pressure was 70 psig. After 4 hours, the HPLC yields of pyruvate and acetate were 97.7% and 1.3%, respectively, and 2.7% lactate remained. The remaining permeabilized cell activity of glycolate oxidase and catalase were 111% and 100%, respectively, of their initial values.

Provenance & Evidence