Oxygen → Acetate

Participants

Reaction Steps

The procedure described in Example 11 was repeated using 100 mL of an aqueous solution containing sodium L-lactate (1.00M) at pH 7.5 (adjusted with 50% NaOH), to which was added 4.99 g (wet weight) of Hansenula polymorpha transformant GO1 (4.12 IU/mL glycolate oxidase and 9,660 IU/mL catalase) which had been permeabilized by treatment with 0.1% benzalkonium chloride ("BARQUAT" MB-50); no buffer was added. The reaction temperature was 5° C. and the oxygen pressure was 70 psig. After 6 hours, the HPLC yields of pyruvate and acetate were 93.5% and 1.5%, respectively, and 4.9% lactate remained. The remaining permeabilized cell activity of glycolate oxidase and catalase were 135% and 99%, respectively, of their initial values.

Provenance & Evidence