Ammonia → Ammonium Aspartate
Reaction equation
[C:1]([OH:8])(=[O:7])/[CH:2]=[CH:3]\[C:4]([OH:6])=[O:5].[NH3:9].C([O-])(=O)/C=C\C([O-])=O.[NH4+].[NH4+]>O>[NH2:9][C@H:2]([C:1]([O-:8])=[O:7])[CH2:3][C:4]([O-:6])=[O:5].[NH4+:9].[NH4+:9]Participants
Reactant 3
Product 1
Reagents, catalysts & solvents 1
Conditions
- Temperature
- 30 °C
Procedure
According to the same procedure of Example 1, 600 g maleic acid was dissolved in deionized water, and 705 g of 25 wt % aqueous ammonia solution (molar ratio to maleic acid was about 2.0) was added thereon. The cells of Alcaligenes faecalis ATCC 8750 were added to 2 L of an ammonium maleate substrate solution (pH 8.4) containing 600 g maleic acid, and the substrate solution were gently stirred at 30° C. for 72 hours. After the reaction, ammonium L-aspartate was formed with a molar yield of 98.8% based on maleic acid. To the reaction solution from which the cells had been removed by centrifugation, was added 610 g maleic acid at 30° C. After the addition, a temperature of the solution was 37.0° C., and pH value thereof was 3.8. L-aspartic acid crystals were separated by filtration, so as to recover 1.5 L of mother liquors. The crude crystals was 769 g (excluding water) (88.0 wt % aspartic acid, 11.4 wt % maleic acid, 250 μm average crystal size). The crystals were washed with about 600 ml of water and dried so as to obtain 675 g of L-aspartic acid crystals (98.8 wt % aspartic acid, 0.77 wt % maleic acid, 100 μm average crystal size).
Workup
- customAfter the reaction
Sources & evidence
- ORD record
- ord-3ae3bf38ed6642a7987f13ef3f67673a
- Source dataset
- uspto-grants-1996_07
- Patent number
- US05541090
Reaction notes
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