反应详情

温度

30 °C

According to the same procedure of Example 6, the cells of Pseudomonas maltophilia ATCC 13270 and the cells of Escherichia coli ATCC 11303 were added to 2 L of an ammonium maleate substrate solution containing 400 g of maleic acid, and the substrate solution was gently stirred at 30° C. for 24 hours. After the reaction, ammonium L-aspartate was formed with a molar yield of 99.3% based on maleic acid. To the reaction solution from which the cells had been removed by centrifugation was added 410 g maleic acid at 30° C. After the addition, a temperature of the solution was 34° C., and pH valve thereof was 3.7. L-aspartic acid crystals were separated by filtration so as to recover 1.6 L of mother liquors. The crude crystals were 517 g (excluding water) (88.0 wt % aspartic acid, 10.7 wt % maleic acid, 400 μm average crystal size). The crystals were washed with about 400 ml of water and dried so as to obtain 452 g (excluding water) of L-aspartic acid crystals (98.6 wt % aspartic acid, 1.0 wt % maleic acid, 300 μm average crystal size).

1. customAfter the reaction