反应详情

To a solution of methyl pheophorbide-a (100 mg, 0.17 mmol) in 2,4,6-collidine (25 mL, distilled) was added distilled water (6 μL, 0.33 mmol, 2 equiv.) and the mixture was refluxed under argon with stirring. The reaction was monitored by HPLC (hexaneacetone, 90:10-mobile phase, LiChrospherCN-column, detection at 425 and 410 nm). After 10 min the reaction was shown to have reached 100% completion by HPLC peak area. The solution was cooled to room temperature after 30 min of reflux and solvent removed under reduced pressure. The residue was precipitated from methylene chloride-methanol to give methyl pyropheophorbide-a (80 mg, 89%).

1. temperaturethe mixture was refluxed under argon
2. temperatureof reflux
3. customsolvent removed under reduced pressure
4. customThe residue was precipitated from methylene chloride-methanol