Water → HCID 178435492, compound

Participants

Reaction Steps

To a solution of methyl pheophorbide-a (100 mg, 0.17 mmol) in DMF (25 mL) was added distilled water (6 μL, 0.33 mmol, 2 equiv.) and the mixture was refluxed under argon with stirring. The reaction was monitored by HPLC (hexane-acetone, 90:10-mobile phase, LiChrospherCN-column, detection at 425 and 410 nm). After 90 min the reaction was shown to have reached 99.5% completion by HPLC peak area. The solution was cooled to room temperature after 110 min of reflux and solvent removed under reduced pressure. The residue was precipitated from methylene chloride-methanol to give methyl pyropheophorbide-a (80 mg, 89%).

Provenance & Evidence