Water → HCID 178435492, compound

Participants

Reaction Steps

To a solution of methyl pheophorbide-a (100 mg, 0.17 mmol) in DMF (25 mL) was added distilled water (48 μL, 2.7 mmol, 16 equiv.) and the mixture was refluxed under argon with stirring. The reaction was monitored by HPLC (hexane-acetone, 90:10-mobile phase, LiChrospherCN-column, detection at 425 and 410 nm). After 60 min the reaction was shown to have reached 99.6% completion by HPLC peak area. The solution was cooled to room temperature after 100 min of reflux and solvent removed under reduced pressure. The residue was precipitated from methylene chloride-methanol to give methyl pyropheophorbide-a (50 mg, 56%).

Provenance & Evidence