HCID 178435494, compound → HCID 178435492, compound

Participants

Reaction Steps

To a solution of methyl pheophorbide-a (100 mg, 0.17 mmol) in 2,4,6-collidine (25 mL, distilled) was added distilled water (1 mL, 56 mmol, 327 equiv., 4% v/v) and the mixture was refluxed under argon with stirring. The reaction was monitored by HPLC (hexane-acetone, 90:10-mobile phase, LiChrospherCN-column, detection at 425 and 410 nm). After 90 min, the reaction was shown to have reached 99.5% completion by HPLC peak area. The solution was cooled to room temperature and solvent removed under reduced pressure. Hexane (10 mL) was added and the precipitated porphyrin was collected by filtration and rinsed with hexane to give methyl pyropheophorbide-a (65 mg, 72%).

Provenance & Evidence