HCID 178435494, compound → HCID 178435492, compound

Participants

Reaction Steps

To a solution of methyl pheophorbide-a (2.0 g, 3.3 mmol) in pyridine (80 mL) was added distilled water (0.8 mL) and the mixture was refluxed under argon with stirring. The reaction was monitored by HPLC (Waters instrument, hexane-acetone, 90:10-mobile phase; LiChrospher CN-column; detection at 410 nm). The HPLC of reaction aliquot after 8.5 h of reflux showed <1% (peak area) of starting methyl pheophorbide-a. The reaction was stopped, cooled to room temperature and solvent removed under reduced pressure. The residue was precipitated from methylene chloride-hexane to give methyl pyropheophorbide-a (1.8 g, 100%).

Provenance & Evidence