L-Glutamine → Ammonia

Participants

Reaction Steps

The polymer of Example 15 is converted to the Glu/Gln co-polymer. Polymeric glutamic acid (20 gm) is suspended in 100 ml o heated to 50° C. Concentrated aqueous ammonia (30%) is added while monitoring the pH of the solution. At pH=5.0, the mixture is stirred for an additional 10 min. while making small adjustments to pH 5.0 and the solution is freeze dried to obtain 20.4 gm of a white powder. The partial ammonium salt is then heated to 80° C. in a vacuum oven for conversion to the Glu/Gln co-polymer. Yield: 18.6 gm. Elemental analysis for N shows this preparation to contain 8% Gln. Titrations to higher pH with ammonia yield higher % Gln in the final co-polymer.

Provenance & Evidence