反应详情

温度

20 °C

To a stirred solution of ethyl 3-(4-(N-isobutyl-N-(2-methyl-5-(trifluoromethyl)phenyl)sulfamoyl)phenyl)-2,2-dimethylpropanoate (15 mg, 0.030 mmol) in tetrahydrofuran (THF) (1.5 mL), at room temperature, was added a solution of lithium hydroxide (approximately 0.719 mg, 0.030 mmol—tip of spatula used) in water (1.5 mL). The reaction mixture was stirred at 20° C. for 4 hours and then stood overnight. The mixture was evaporated in vacuo, then partitioned between water (2 mL) and ethyl acetate (2×5 mL). The organic fraction was separated (hydrophilic frit) and evaporated in vacuo to give a residue. This was redissolved in THF (1 mL) and water (1 mL) and new lithium hydroxide (tip of spatula) was added. The reaction was stood for 3 days, after which time the solvents had evaporated to give a white residue. The residue was acidified (2N HCl, 3 mL) and extracted with ethyl acetate (2×5 mL). The organics were separated (hydrophilic frit) and concentrated in vacuo, then purified by MDAP (Method F) to give the required product, 2.38 mg. LCMS (M+1) 472, RT 1.37 mins.

1. waitstood overnight
2. customThe mixture was evaporated in vacuo
3. custompartitioned between water (2 mL) and ethyl acetate (2×5 mL)
4. customThe organic fraction was separated (hydrophilic frit)
5. customevaporated in vacuo
6. customto give a residue
7. waitThe reaction was stood for 3 days
8. customafter which time the solvents had evaporated
9. customto give a white residue
10. extractionextracted with ethyl acetate (2×5 mL)
11. customThe organics were separated (hydrophilic frit)
12. concentrationconcentrated in vacuo
13. custompurified by MDAP (Method F)