5,5'-Dithiobis(2-nitrobenzoic Acid) → Diglyceryl silane

참여 물질

반응 단계

Sodium silicate solution (˜14% NaOH, ˜27% SiO2), tetraethylorthosilicate (TEOS, 98%), Dowex 50WX8-100 ion-exchange resin, acetylcholinesterase (AChE, from electrophorus electricus, EC 3.1.1.7), paraoxon, aflatoxin B1 (AfB1, from aspergillus flavus), 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB), carboxymethylcellulose sodium salt (CMC), and Triton X-100 were obtained from Sigma-Aldrich. Anhydrous glycerol and acetylthiocholine iodide (ATCh) were purchased from Fluka BioChemika Ultra (UK). Diglyceryl silane (DGS) was synthesized in our lab using by transesterification of TEOS with anhydrous glycerol as described in detail elsewhere.26 Polyvinylamine (PVAm; 1.5 MDa) was obtained from BASF (Mississauga, Canada), as a gift. Mead brand cardboard paper substrate with a white hydrophobic clay coating (Manufactured by Hilroy, Toronto, Canada) was purchased from McMaster University Bookstore. Distilled deionized water (ddH2O) was obtained from a Milli-Q Synthesis A10 water purification system. All other reagents were of analytical grade.

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