Glycolic Acid → Glyoxylic Acid

Participants

Reaction Steps

The reaction in Example 4 was repeated using 10 mL of a solution containing glycolic acid (0.75M), ethylenediamine (0.86M), propionic acid (0.075M, HPLC internal standard), flavin mononucleotide (0.2 mM), 2.5 IU of spinach glycolate oxidase immobilized on Eupergit C, and 14,000 IU of Aspergillus niger catalase immobilized on Eupergit C (the two enzymes were not co-immobilized on the same support). After 20 hours, the yields of glyoxylic acid, oxalic acid, and formic acid were 99%, 0.2%, and 0.5%, respectively, with a 100% conversion of glycolic acid. The final activities of glycolate oxidase and catalase were 72% and 66% of their initial values, respectively.

Provenance & Evidence