Cholesterol sulfate → HCID 178443926, compound

HRID 1761836Notes

Reaction equation

HRID 1761836 reaction equation
[S:1]([O:5][C@H:6]1[CH2:30][CH2:29][C@@:28]2([CH3:31])[C:8](=[CH:9][CH2:10][C@@H:11]3[C@@H:27]2[CH2:26][CH2:25][C@@:24]2([CH3:32])[C@H:12]3[CH2:13][CH2:14][C@@H:15]2[C@H:16]([CH3:23])[CH2:17][CH2:18][CH2:19][CH:20]([CH3:22])[CH3:21])[CH2:7]1)([OH:4])(=[O:3])=[O:2].CO.C(Cl)(Cl)Cl.O.[CH:40]1[C:41]([NH2:54])=[N+:42]([O-:53])[C:43]([NH2:52])=[N:44][C:45]=1[N:46]1[CH2:51][CH2:50][CH2:49][CH2:48][CH2:47]1>C(Cl)(Cl)Cl>[CH:40]1[C:41]([NH2:54])=[N+:42]([O-:53])[C:43]([NH2:52])=[N:44][C:45]=1[N:46]1[CH2:51][CH2:50][CH2:49][CH2:48][CH2:47]1.[S:1]([O:5][C@H:6]1[CH2:30][CH2:29][C@@:28]2([CH3:31])[C:8](=[CH:9][CH2:10][C@@H:11]3[C@@H:27]2[CH2:26][CH2:25][C@@:24]2([CH3:32])[C@H:12]3[CH2:13][CH2:14][C@@H:15]2[C@H:16]([CH3:23])[CH2:17][CH2:18][CH2:19][CH:20]([CH3:22])[CH3:21])[CH2:7]1)([OH:4])(=[O:2])=[O:3]

Participants

5

Reactant 3

HCID 65076Cholesterol sulfateHCID 21866399Chloroform methanol waterHCID 178507853HCID 178507853, compound2×

Product 1

HCID 178443926HCID 178443926, compound

Reagents, catalysts & solvents 1

HCID 6212ChloroformSolvent

Procedure

Cholesterol sulfate was converted to the free acid form by cation exchange chromatography in a methanol/chloroform/water (5:4:1) solvent. The free acid form (120 mgs) was dissolved in 2 ml of chloroform, and this solutions was added to a round bottom flask containing 40 mg minoxidil and 370 mgs of PC. The flask was gently agitated until the components were completely dissolved. The solution was dried to a thin lipid film in a round bottom flask with rotary evaporation and lyophilization, as above. A liposome suspension was prepared as in Example 8. No minoxidil crystal were observed in the suspension.

Workup

  1. additionthis solutions was added to a round bottom flask
  2. dissolutionwere completely dissolved
  3. customThe solution was dried to a thin lipid film in a round bottom flask with rotary evaporation and lyophilization
  4. customA liposome suspension was prepared as in Example 8

Sources & evidence

ORD record
ord-e8be45aa609d441d8789a7fb6f9ac08b
Source dataset
uspto-grants-1989_05
Patent number
US04828837

Reaction notes

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