Acetone → Tetrahydrocannabinol

Participants

Reaction Steps

One hundred mg of human IgG were dissolved in 7 ml of 0.2M NaHCO3, pH 9.6, and 108 mg of a cannabinol derivative having the formula ##STR5## in 2 ml of dioxane were added. Nine additional ml of dioxane were added, and the mixture was mixed intermittently for 4 hours at 4° C., after which 180 ml of cold acetone were added. The mixture was centrifuged for 10 minutes at 2,000×g, and the pellet was washed once with 20 ml of cold acetone. Following centrifugation for 10 minutes at 2,000×g, the pellet was resuspended in 10 ml of phosphate buffered saline (PBS). Dialysis was carried out for 3 days at 4° C. against 2 changes per day of 1000-ml volumes of PBS, to produce a THC-human IgG conjugate.

Provenance & Evidence