Sodium Citrate Dihydrate → Trisodium citrate

Participants

Reaction Steps

Ten mg of EDTA were added to a clean test tube. Ten mg of HEDTA were added to a second clean test tube. Ten mg each of NTA, EBT, ADA, and MDA were added to third, fourth, fifth, and sixth test tubes. Twenty mg of sodium citrate dihydrate dissolved in 0.5 ml of water were added to a seventh clean test tube. Blood from a single donor was collected into each tube. The volume of blood collected into each of the first six tubes was 7 ml and the volume of blood collected into the seventh tube was 4.5 ml. All the tubes were stoppered and inverted gently 10 times to mix the contents. The tubes were allowed to stand for 30 minutes so that clotting of blood could take place in the tubes that did not contain true in vitro anticoagulants. After the contents of the tubes were mixed again, whole blood samples were removed when possible, to determine hematocrit and platelet count, and to make a blood smear for staining and microscopic examination of cell morphology. The whole blood samples were then centrifuged to obtain plasma for use in the coagulation determinations of prothrombin time and activated partial thromboplastin time. The results are tabulated in Table I.

Provenance & Evidence