HuagongsheHuagongshe Chemistry Print Report · For research reference only
Kanamycin → (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | Kanamycin | HCID 6032 | — |
| Reactant | L-Phenylalanine | HCID 6140 | — |
| Reactant | Ampicillin | HCID 6249 | — |
| Reactant | Pca 16 | HCID 131037 | — |
| Product | (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal | HCID 107526 | — |
Reaction Steps
Two strains (designated as WWQ51.1 and WSQ1) were constructed from the E. coli phenylalanine overproducing strain E. coli NST74 by transformation with pCA16 and pKSM-pdc1 and selection for ampicillin and kanamycin resistance, as described in Example 4. Since preliminary experiments showed similar patterns for both constructs, we chose strain WWQ51.1 for further studies.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US07229806B2 |
| Source dataset | uspto-grants-2007_06 |
| ORD ID | ord-020b9a2552d84b3cbea21ca7de791de7 |
| Source note | uspto-grants-2007_06 |