Phosphate → beta-D-Galactopyranose

Participants

Reaction Steps

To the Fab' (1 mg) prepared in the above process (a) was added the β-Gal-maleimide (3.9 mg) prepared in the process (b) and the mixture was reacted at 30° C. for 30 minutes. The reaction solution was centrifuged and the supernatant was subjected to Sepharose 6B column (φ1.5 cm×50 cm, eluent: 10 mM phosphate buffer, pH 6.5, containing 0.1% BSA) to give a β-Gal-Fab' fragment.

Provenance & Evidence