Hydrazine hydrate (1:1) → HCID 54075093, compound

Participants

Reaction Steps

A solution of 100 mg 3-PHT-E-AZT (Example 25) in 1 ml absolute EtOH was treated with 25 ul hydrazine hydrate, incubated overnight at room temperature and chromatographed by preparative TLC on silica gel-F 1000 um using the solvent system CHCl3 /MeOH/15M NH4OH (90+10+1). The UV positive band at Rf =0.25 was eluted with MeOH. Analytical TLC of the methanol extract in the same solvent system with silica gel-F 250 um showed the product to be homogeneous and ninhydrin positive. In this system a hydrazine reference migrated close to the origin as a UV negative, ninhydrin positive spot and reference phthalhydrazide remained at the origin as a UV positive, ninhydrin negative spot.

Provenance & Evidence