HuagongsheHuagongshe Chemistry Print Report · For research reference only
Alpha-Ketoglutarate → Oxalacetate
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | Alpha-Ketoglutarate | HCID 164533 | — |
| Reactant | l-aspartic acid, sodium salt | HCID 5460541 | — |
| Product | Oxalacetate | HCID 164550 | — |
| Product | L-glutamate(2-) | HCID 5460544 | — |
Reaction Steps
PRINCIPLE ##STR5## AST catalyzes the transfer of the amino group from aspartate to 2-oxoglutarate to yield oxalacetate and glutamate. The oxalacetate is reduced to malate in the presence of malate dehydrogenase [MD] with the simultaneous oxidation of reduced nicotinamide adenine dinucleotide [NADH]. The rate of decrease in absorbance at 340 nm is directly proportional to AST activity.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US05382525 |
| Source dataset | uspto-grants-1995_01 |
| ORD ID | ord-01a7a9d20ed64ae891a8cf0d3bd44d71 |
| Source note | uspto-grants-1995_01 |