14919-35-8 → (-)-Carnitine

Participants

Reaction Steps

Octanoyl-L-carnitine solutions, in 100 mM acetate buffer solution (pH 4.5-6.0), in 100 mM phosphate buffer solution (pH 6.5-8.0), in 100 mM tris-HCl buffer solution (pH 8.0-9.0) and in 100 mM glycine/NaOH buffer solution (pH 9.0-10.0) were prepared with a concentration of octanoyl-carnitine of 0.5 mM for each of the solutions. Each of these solutions was subjected to an enzymatic hydrolysis reaction at 37° C. for 10 hours and the amount of L-carnitine formed thereby was determined. The results of the analysis of L-carnitine are shown on the graph of FIG. 1, which shows the optimum pH at about 8.0, wherein high enzymatic activities above 90% were reached within a wide pH range from 6.5 to 9.5.

Provenance & Evidence