反应详情

The reaction was carried out in a reaction mixture (30 ml), comprising 500 mM 3-cyanopyridine, 40 mM potassium phosphate buffer (pH 7.0) and resting cells (dry weight 2.3 mg). During the reaction, 3-cyanopyridine (500 mM) was added 7 times as soon as it was consumed. In this manner, 4.0 M 3-cyanopyridine was added in the course of 15 h and 3.89 M (475 g/l) nicotinamide was formed, corresponding to a yield of 97.3%. Nicotinic acid was not formed.

1. customcells (dry weight 2.3 mg)
2. customDuring the reaction, 3-cyanopyridine (500 mM)
3. additionwas added 7 times as soon as it
4. customwas consumed
5. additionIn this manner, 4.0 M 3-cyanopyridine was added in the course of 15 h