Lithium Hydroxide → HCID 118157506, compound

Participants

Reaction Steps

No. 5250270, (E)-(2S,3S)-3-[(S)-2-(4-but-2-ynyloxy-phenyl)-1-methoxycarbonyl-ethylcarbamoyl]-2-cyanomethyl-2-hydroxy-12-oxo-nonadec-4-enoic acid (81 mg, 0.127 mmol) was dissolved in methanol (4.0 mL), and 2 M LiOH (0.40 mL) was added. The mixture was stirred at room temperature. After confirming the consumption of the starting materials by LCMS, 1 M hydrochloric acid was added, and the mixture was extracted with ethyl acetate. The resulting organic layer was washed with a saturated brine and then dried over anhydrous sodium sulfate. The filtrate was concentrated under reduced pressure. The resulting residue was purified by preparative HPLC and freeze-dried to obtain the title compound (11 mg, 14% yield, yellow oil).

Provenance & Evidence