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HRID1779736

反应详情

EQUATION

反应方程式

HRID 1779736 的结构方程式

REACTANTS

反应物

5
结构：[Na+].[OH-]
HCID14798

Sodium Hydroxide

HNaO

结构：[N-]=[N+]=[N-].[Na+]
HCID33557

未命名化合物

结构：CC(=O)N[C@H]1[C@H]([C@H](O)[C@H](O)CO)OC(O)(C(=O)O)C[C@@H]1O
HCID439197

N-Acetyl-Neuraminic Acid

C11H19NO9

结构：CC(=O)N[C@H]1C(O)O[C@H](CO)[C@@H](F)[C@@H]1O
HCID10466144

未命名化合物

结构：CC(=O)C(=O)[O-].[Na+]
HCID23662274

Sodium pyruvate

C3H3NaO3

PRODUCTS

生成物

1
结构：CC(=O)N[C@H]1[C@H]([C@H](F)[C@H](O)CO)OC(O)(C(=O)O)C[C@@H]1O
HCID102244862

未命名化合物

收率 19.2%

AUXILIARIES

试剂、催化剂与溶剂

1
结构：O
溶剂HCID962

Water

H2O

CONDITIONS

反应条件

温度
20 °C

PROCEDURE

实验过程

The compound (16) (1.241 g), sodium pyruvate (1.200 g) and sodium azide (4.3 mg) were dissolved in distilled water (4.3 ml) and the pH of the resulting solution was adjusted to 10.59 by 2N sodium hydroxide. To this solution, 4.3 mg (112 U) of N-acetyl-neuraminic acid aldolase (NAL-300, manufactured by Toyobo Co., Ltd.) was added, followed by stirring mildly at 20° C. for 4 days. Thereafter, the reaction solution was desalted with an ion exchange resin (Dowex 50X8-200, H-type) and purified by an ion exchange chromatography (Dowex 1, HCOOH-type; aqueous 1.0M-HCOOH solution) to give 0.342 g of the candy-like titled compound (yield: 19.2%).

WORKUP

后处理

  1. addition) was added
  2. custompurified by an ion exchange chromatography (Dowex 1, HCOOH-type; aqueous 1.0M-HCOOH solution)