Morpholine → HCID 70049647, compound

Participants

Reaction Steps

A solution of 47 mg (0.060 mmol) of 1-[(2S,3S)-3-{N2 -[4-(P-N-nitrophenoxycarbonyl-N-methylamino)-phenoxy]acetyl-L-asparaginyl}amino-2-hydroxy-4-phenylbutyryl]-N-t-butyl-L-prolinamide (prepared as described in Example 42) dissolved in 0.5 ml of morpholine was allowed to stand at room temperature for 6 days. At the end of this time, the reaction mixture was freed from an excess of morpholine by distillation under reduced pressure, and the residue was diluted with chloroform and then washed with water. The organic layer was dried over anhydrous sodium sulfate and the solvent was removed by distillation under reduced pressure. The residue was purified by preparative thin layer chromatography, using a 8:1 by volume mixture of methylene chloride and methanol as the developing solvent, to give 30 mg of the title compound as a colorless powder, melting at 112°-114° C.

Provenance & Evidence